Zhipeng Yu 1,2†Tianting Zhong 1,2†Huanhao Li 1,2Haoran Li 1,2[ ... ]Puxiang Lai 1,2,6,8,*
Author Affiliations
Abstract
1 Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong SAR, China
2 Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen 518000, China
3 Peng Cheng Laboratory, Shenzhen 518055, China
4 Key Laboratory for Quantum Optics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
5 Department of Electronic and Information Engineering, The Hong Kong Polytechnic University, Hong Kong SAR, China
6 Photonics Research Institute, The Hong Kong Polytechnic University, Hong Kong SAR, China
7 e-mail: chao.lu@polyu.edu.hk
8 e-mail: puxiang.lai@polyu.edu.hk
Multimode fibers (MMFs) are a promising solution for high-throughput signal transmission in the time domain. However, crosstalk among different optical modes within the MMF scrambles input information and creates seemingly random speckle patterns at the output. To characterize this process, a transmission matrix (TM) can be used to relate input and output fields. Recent innovations use TMs to manipulate the output field by shaping the input wavefront for exciting advances in deep-brain imaging, neuron stimulation, quantum networks, and analog operators. However, these approaches consider input/output segments as independent, limiting their use for separate signal processing, such as logic operations. Our proposed method, which makes input/output segments as interdependent, adjusts the phase of corresponding output fields using phase bias maps superimposed on input segments. Coherent superposition enables signal logic operations through a 15-m-long MMF. In experiments, a single optical logic gate containing three basic logic functions and cascading multiple logic gates to handle binary operands is demonstrated. Bitwise operations are performed for multi-bit logic operations, and multiple optical logic gates are reconstructed simultaneously in a single logic gate with polarization multiplexing. The proposed method may open new avenues for long-range logic signal processing and transmission via MMFs.
Photonics Research
2024, 12(3): 587
赖溥祥 1,2,3,4,*赵麒 1,2周颖颖 1,2程圣福 1,2[ ... ]仲天庭 1,2,**
作者单位
摘要
1 香港理工大学生物医学工程系,香港 九龙999077
2 香港理工大学深圳研究院,广东 深圳 518055
3 香港理工大学光子技术研究院,香港 九龙999077
4 香港理工大学体育科技研究院,香港 九龙999077

光学技术在生物医学中扮演着越来越重要的角色,其非电离辐射、高分辨率、高对比度和对生物组织异变高度灵敏等特性使其非常适用于生物组织的研究,包括成像、传感、治疗、刺激以及控制等。然而由于光折射因子在生物组织中的分布是不均匀的,光在生物组织中的传播会受到很强的散射影响,故纯光学技术的穿透深度和空间分辨率是“鱼和熊掌不可兼得”;高分辨率光学成像应用仅限于样品浅表层,当成像深度增加时分辨率急剧下降。实现光在深层生物组织里的高分辨率成像或应用是人们期盼已久的目标。近年来,为解决这一问题,研究者提出了不同的方法,例如切换到更长的光波长以减小组织散射系数,在信号检测时将漫射光转换为散射不明显的超声信号,逆转或者预先补偿由光的多次散射所带来的相位畸变,或借助光纤等微创光学通道实现深层生物组织的高分辨率光学成像、刺激等。基于团队在深层生物组织光学相关领域多年的耕耘,从光在生物组织中的传播特性出发,梳理和总结了近年来研究人员在光-声结合和光学波前整形技术等方面展开的诸多探索,以及在生物组织操控、成像、光学计算以及人工智能等领域中的应用尝试。虽然尚有诸多不足,但随着硬件设备的更新和计算技术的发展,在不远的将来有望实现活体深层生物组织光学高分辨率应用。在这一求索过程中,新方法和新能力将不断激发新的应用灵感,为光学尤其是生物医学光子学带来全新的理念和机遇。

生物光学 光学成像 生物医学光子学 深层组织 光学波前整形 光声成像 
中国激光
2024, 51(1): 0107003
Shengfu Cheng 1,2†Xuyu Zhang 3,4Tianting Zhong 1,2Huanhao Li 1,2[ ... ]Puxiang Lai 1,2,7,*
Author Affiliations
Abstract
1 The Hong Kong Polytechnic University, Department of Biomedical Engineering, Hong Kong, China
2 The Hong Kong Polytechnic University, Shenzhen Research Institute, Shenzhen, China
3 Chinese Academy of Sciences, Shanghai Institute of Optics and Fine Mechanics, Key Laboratory for Quantum Optics, Shanghai, China
4 University of Shanghai for Science and Technology, School of Optical-Electrical and Computer Engineering, Shanghai, China
5 University of Science and Technology of China, Department of Optics and Optical Engineering, Hefei, China
6 University of Chinese Academy of Sciences, Center of Materials Science and Optoelectronics Engineering, Beijing, China
7 The Hong Kong Polytechnic University, Photonics Research Institute, Hong Kong, China
Transmission matrix (TM) allows light control through complex media, such as multimode fibers (MMFs), gaining great attention in areas, such as biophotonics, over the past decade. Efforts have been taken to retrieve a complex-valued TM directly from intensity measurements with several representative phase-retrieval algorithms, which still see limitations of slow or suboptimum recovery, especially under noisy environments. Here, we propose a modified nonconvex optimization approach. Through numerical evaluations, it shows that the optimum focusing efficiency is approached with less running time or sampling ratio. The comparative tests under different signal-to-noise levels further indicate its improved robustness. Experimentally, the superior focusing performance of our algorithm is collectively validated by single- and multispot focusing; especially with a sampling ratio of 8, it achieves a 93.6% efficiency of the gold-standard holography method. Based on the recovered TM, image transmission through an MMF is realized with high fidelity. Due to parallel operation and GPU acceleration, our nonconvex approach retrieves a 8685 × 1024 TM (sampling ratio is 8) with 42.3 s on average on a regular computer. The proposed method provides optimum efficiency and fast execution for TM retrieval that avoids the need for an external reference beam, which will facilitate applications of deep-tissue optical imaging, manipulation, and treatment.
transmission matrix phase retrieval multimode fiber imaging wavefront shaping 
Advanced Photonics Nexus
2023, 2(6): 066005
Author Affiliations
Abstract
1 Hong Kong Polytechnic University, Department of Biomedical Engineering, Hong Kong, China
2 Hong Kong Polytechnic University, Shenzhen Research Institute, Shenzhen, China
3 Hong Kong Polytechnic University, Photonics Research Institute, Hong Kong, China
Controllable optical propagation, such as forming diffraction-limited optical focusing, beyond the diffusion limit in biological tissue or tissue-like scattering media, has been desired for long yet considered challenging. In the past two decades, optical wavefront shaping (WFS) has been proposed and has progressed, demonstrating its remarkable potential. That said, inherent tradeoffs still exist among optimization speed, control degree of freedom, and energy gain, which has hindered wide applications of the technology. Most recently, an analogue optical phase conjugation system was developed, equipped with stimulated emission light amplification that effectively achieves the least tradeoff ever, yielding high-gain and high-speed performance of optical focusing through dynamic thick media.
Advanced Photonics
2023, 5(2): 020502
Huanhao Li 1,2†Zhipeng Yu 1,2†Qi Zhao 1,2†Yunqi Luo 3[ ... ]Puxiang Lai 1,2,6,9,*
Author Affiliations
Abstract
1 Department of Biomedical Engineering, Hong Kong Polytechnic University, Hong Kong, China
2 Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen 518063, China
3 School of Electrical and Electronics Engineering, Nanyang Technological University, Singapore 639798, Singapore
4 Key Laboratory for Quantum Optics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
5 Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, California Institute of Technology, Pasadena, California 91125, USA
6 Photonics Research Institute, Hong Kong Polytechnic University, Hong Kong, China
7 e-mail: LVW@caltech.edu
8 e-mail: yjzheng@ntu.edu.sg
9 e-mail: puxiang.lai@polyu.edu.hk
Information retrieval from visually random optical speckle patterns is desired in many scenarios yet considered challenging. It requires accurate understanding or mapping of the multiple scattering process, or reliable capability to reverse or compensate for the scattering-induced phase distortions. In whatever situation, effective resolving and digitization of speckle patterns are necessary. Nevertheless, on some occasions, to increase the acquisition speed and/or signal-to-noise ratio (SNR), speckles captured by cameras are inevitably sampled in the sub-Nyquist domain via pixel binning (one camera pixel contains multiple speckle grains) due to finite size or limited bandwidth of photosensors. Such a down-sampling process is irreversible; it undermines the fine structures of speckle grains and hence the encoded information, preventing successful information extraction. To retrace the lost information, super-resolution interpolation for such sub-Nyquist sampled speckles is needed. In this work, a deep neural network, namely SpkSRNet, is proposed to effectively up sample speckles that are sampled below 1/10 of the Nyquist criterion to well-resolved ones that not only resemble the comprehensive morphology of original speckles (decompose multiple speckle grains from one camera pixel) but also recover the lost complex information (human face in this study) with high fidelity under normal- and low-light conditions, which is impossible with classic interpolation methods. These successful speckle super-resolution interpolation demonstrations are essentially enabled by the strong implicit correlation among speckle grains, which is non-quantifiable but could be discovered by the well-trained network. With further engineering, the proposed learning platform may benefit many scenarios that are physically inaccessible, enabling fast acquisition of speckles with sufficient SNR and opening up new avenues for seeing big and seeing clearly simultaneously in complex scenarios.
Photonics Research
2023, 11(4): 631
Yunqi Luo 1†Suxia Yan 1†Huanhao Li 2,3†Puxiang Lai 2,3,4,*Yuanjin Zheng 1,5,*
Author Affiliations
Abstract
1 School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore
2 Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong SAR, China
3 The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen 518034, China
4 e-mail: puxiang.lai@polyu.edu.hk
5 e-mail: yjzheng@ntu.edu.sg

Optical focusing through scattering media is of great significance yet challenging in lots of scenarios, including biomedical imaging, optical communication, cybersecurity, three-dimensional displays, etc. Wavefront shaping is a promising approach to solve this problem, but most implementations thus far have only dealt with static media, which, however, deviates from realistic applications. Herein, we put forward a deep learning-empowered adaptive framework, which is specifically implemented by a proposed Timely-Focusing-Optical-Transformation-Net (TFOTNet), and it effectively tackles the grand challenge of real-time light focusing and refocusing through time-variant media without complicated computation. The introduction of recursive fine-tuning allows timely focusing recovery, and the adaptive adjustment of hyperparameters of TFOTNet on the basis of medium changing speed efficiently handles the spatiotemporal non-stationarity of the medium. Simulation and experimental results demonstrate that the adaptive recursive algorithm with the proposed network significantly improves light focusing and tracking performance over traditional methods, permitting rapid recovery of an optical focus from degradation. It is believed that the proposed deep learning-empowered framework delivers a promising platform towards smart optical focusing implementations requiring dynamic wavefront control.

Photonics Research
2021, 9(8): 0800B262
Huanhao Li 1,2†Chi Man Woo 1,2†Tianting Zhong 1,2Zhipeng Yu 1,2[ ... ]Puxiang Lai 1,2,6,*
Author Affiliations
Abstract
1 Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong SAR, China
2 The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, China
3 School of Electrical and Electronics Engineering, Nanyang Technological University, Singapore, Singapore
4 CAS Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing, China
5 e-mail: hui.hui@ia.ac.cn
6 e-mail: puxiang.lai@polyu.edu.hk
Optical imaging through or inside scattering media, such as multimode fiber and biological tissues, has a significant impact in biomedicine yet is considered challenging due to the strong scattering nature of light. In the past decade, promising progress has been made in the field, largely benefiting from the invention of iterative optical wavefront shaping, with which deep-tissue high-resolution optical focusing and hence imaging becomes possible. Most of the reported iterative algorithms can overcome small perturbations on the noise level but fail to effectively adapt beyond the noise level, e.g., sudden strong perturbations. Reoptimizations are usually needed for significant decorrelation to the medium since these algorithms heavily rely on the optimization performance in the previous iterations. Such ineffectiveness is probably due to the absence of a metric that can gauge the deviation of the instant wavefront from the optimum compensation based on the concurrently measured optical focusing. In this study, a square rule of binary-amplitude modulation, directly relating the measured focusing performance with the error in the optimized wavefront, is theoretically proved and experimentally validated. With this simple rule, it is feasible to quantify how many pixels on the spatial light modulator incorrectly modulate the wavefront for the instant status of the medium or the whole system. As an example of application, we propose a novel algorithm, the dynamic mutation algorithm, which has high adaptability against perturbations by probing how far the optimization has gone toward the theoretically optimal performance. The diminished focus of scattered light can be effectively recovered when perturbations to the medium cause a significant drop in the focusing performance, which no existing algorithms can achieve due to their inherent strong dependence on previous optimizations. With further improvement, the square rule and the new algorithm may boost or inspire many applications, such as high-resolution optical imaging and stimulation, in instable or dynamic scattering environments.
Photonics Research
2021, 9(2): 02000202
Zihao Li 1,2,5†Zhipeng Yu 1,2†Hui Hui 3†Huanhao Li 1,2[ ... ]Puxiang Lai 1,2,*
Author Affiliations
Abstract
1 Deparment of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong SAR, China
2 The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen 518000, China
3 CAS Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China
4 Key Laboratory for Quantum Optics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
5 Currently at: Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Edge enhancement is a fundamental and important topic in imaging and image processing, as perception of edge is one of the keys to identify and comprehend the contents of an image. Edge enhancement can be performed in many ways, through hardware or computation. Existing methods, however, have been limited in free space or clear media for optical applications; in scattering media such as biological tissue, light is multiple scattered, and information is scrambled to a form of seemingly random speckles. Although desired, it is challenging to accomplish edge enhancement in the presence of multiple scattering. In this work, we introduce an implementation of optical wavefront shaping to achieve efficient edge enhancement through scattering media by a two-step operation. The first step is to acquire a hologram after the scattering medium, where information of the edge region is accurately encoded, while that of the nonedge region is intentionally encoded with inadequate accuracy. The second step is to decode the edge information by time-reversing the scattered light. The capability is demonstrated experimentally, and, further, the performance, as measured by the edge enhancement index (EI) and enhancement-to-noise ratio (ENR), can be controlled easily through tuning the beam ratio. EI and ENR can be reinforced by 8.5 and 263 folds, respectively. To the best of our knowledge, this is the first demonstration that edge information of a spatial pattern can be extracted through strong turbidity, which can potentially enrich the comprehension of actual images obtained from a complex environment.
Photonics Research
2020, 8(6): 06000954
Author Affiliations
Abstract
1 Department of Biomedical Engineering, Hong Kong Polytechnic University, Hong Kong, P. R. China
2 Shenzhen Research Institute,Hong Kong Polytechnic University, Shenzhen 518057, P. R. China
3 Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, P. R. China
Wavefront shaping (WFS) techniques have been used as a powerful tool to control light propagation in complex media, including multimode fibers. In this paper, we propose a new application of WFS for multimode fiber-based sensors. The use of a single multimode fiber alone, without any special fabrication, as a sensor based on the light intensity variations is not an easy task. The twist effect on multimode fiber is used as an example herein. Experimental results show that light intensity through the multimode fiber shows no direct relationship with the twist angle, but the correlation coe±cient (CC) of speckle patterns does. Moreover, if WFS is applied to transform the spatially seemingly random light pattern at the exit of the multimode fiber into an optical focus. The focal pattern correlation and intensity both can serve to gauge the twist angle, with doubled measurement range and allowance of using a fast point detector to provide the feedback. With further development, WFS may find potentials to facilitate the development of multimode fiber-based sensors in a variety of scenarios.
Multimode fiber wavefront shaping scattering media fiber sensor optical focusing 
Journal of Innovative Optical Health Sciences
2019, 12(4): 1942007
Author Affiliations
Abstract
1 Department of Biomedical Engineering, Hong Kong Polytechnic University, Hong Kong SAR
2 College of Material Science and Engineering, Sichuan University, Sichuan, P. R. China
3 School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore
Coherent optical control within or through scattering media via wavefront shaping has seen broad applications since its invention around 2007. Wavefront shaping is aimed at overcoming the strong scattering, featured by random interference, namely speckle patterns. This randomness occurs due to the refractive index inhomogeneity in complex media like biological tissue or the modal dispersion in multimode fiber, yet this randomness is actually deterministic and potentially can be time reversal or precompensated. Various wavefront shaping approaches, such as optical phase conjugation, iterative optimization, and transmission matrix measurement, have been developed to generate tight and intense optical delivery or high-resolution image of an optical object behind or within a scattering medium. The performance of these modulations, however, is far from satisfaction. Most recently, artificial intelligence has brought new inspirations to this field, providing exciting hopes to tackle the challenges by mapping the input and output optical patterns and building a neuron network that inherently links them. In this paper, we survey the developments to date on this topic and briefly discuss our views on how to harness machine learning (deep learning in particular) for further advancements in the field.
Optical scattering deep learning wavefront shaping adaptive optics computational imaging 
Journal of Innovative Optical Health Sciences
2019, 12(4): 1930006

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